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The peptides mechanism relies on sustained signaling to trigger lasting gene expression changes

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Sample lysates were separated via 414% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), following which the proteins were transferred to polyvinylidene difluoride (PVDF) membranes

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This reduction in NAD + was accompanied by decreased SIRT1 mRNA levels, suggesting that SYNCRIP may regulate both the abundance and functional capacity of SIRT1 (Figure S4I)