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cDNAs of tissues total RNA were synthesized using a PrimerScript TM RT reagent Kit with a gDNA Eraser (Perfect Real Time)(Takara, Japan) by S1000 TM Thermal Cycler (Bio-Rad, USA)

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Pathway 2: Nitric Oxide System Modulation Independently, the BPC-157 peptide interacts with the nitric oxide (NO) signaling system a key regulator of vascular tone, inflammation resolution, and cellular repair signaling