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Biochim Biophys Acta 268:305312 Terai Y, Ueno H, Ogawa T, Sawa Y, Miyagi A, Kawai-Yamada M, Ishikawa T, Maruta T (2020) Dehydroascorbate reductases and glutathione set a threshold for high-lightinduced ascorbate accumulation

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doi:10.1093/annonc/mdg448

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To monitor the transfer of the glutathione moiety from PfGrx to roGFP2, 5 M reduced roGFP2 WT , roGFP2 C151S or roGFP2 C208S were mixed with variable concentrations of glutathionylated PfGrx C88S or PfGrx C32S/C88S at room temperature for 1 h