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Blood was first diluted 1:1 in PBS, and PBMCs were isolated using SepMate-50 separation tubes (STEMCELL Technologies) according to the manufacturers instructions

Immediately, 556ul of 37% formaldehyde was added to each dish to reach a final concentration of 1%

Muscle cells aren't the only cells that work more efficiently in the presence of carnitine

The lethal dose threshold (LD1) was never achieved in animal safety studies meaning researchers could not identify a dose lethal to 1% of test subjects

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The protein content of the supernatant was determined using the Bradford method