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Interestingly, exogenous HGF did not promote spherogenicity under these culture conditions, however, profound induction of sphere formation was seen when EGF and FGF were omitted from the medium in ZH-161 and ZH-305 except T-269

[DOI] [PMC free article] [PubMed] 37.Wang L, Wang W, Kaelber DC, Xu R, Berger NA

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Basic conditions were achieved by running buffer C (50 mM ammonium hydroxide) constantly at 10% (100 ml/min, 2.5 mM ammonium bicarbonate final)