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These results support the conclusion that the sets of identified Gcg , Gcgr , Glp1r , and Glp2r gene sequences identified here are orthologs

Definitive quality verification requires analytical HPLC showing a single dominant peak with greater than 95% area purity and electrospray mass spectrometry confirming the intact molecular ion at m/z 1024.18, with the absence of +16 Da oxidation adducts or -17 Da deamidation products that indicate compromised peptide integrity