These results are consistent with the major features of hind-limb suspension-related phenotypes of skeletal muscles as an index of anti-gravity muscle atrophy 66 , thus assuring that the experimental design including the mouse age and muscle-disuse period (in vivo assay time-point) were appropriate to the conclusion that 3-days LASSS oral-administration prevents tyrosine-residue nitration of extracellular HGF (Y198) during 5-days muscle disuse
Watanabe et al., 2002)
Frankel EN, Waterhouse AL, Kinsella JE
Classen AT, Sundqvist MK, Henning JA, Newman GS, Moore JA, Cregger MA, Moorhead LC, Patterson CM (2015) Direct and indirect effects of climate change on soil microbial and soil microbial-plant interactions: what lies ahead
Thus, this pilot study has laid a foundation for the successful implementation of immunogenicity and efficacy vaccination trials in larger cohorts in Asian elephant range countries and may provide a roadmap for vaccination studies of other wildlife diseases
Then, probe for secondary contributors, reconsider age-adjusted reference ranges, and assess clinical signs
