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For the phosphorylated proteins, 1,000 l each of SuperSignal West Femto Luminol/Enhancer Solution (Thermo Scientific) and SuperSignal West Femto Stable Peroxide Buffer (Thermo Scientific) were mixed and pipetted onto the nitrocellulose membrane

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The "Understanding Pyoderma Gangrenosum, Review and Assessment of Disease Effects (UPGRADE)" Project: a protocol for the development of the core outcome domain set for trials in pyoderma gangrenosum

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