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The extensive breadth of these different molecules highlights the diversity of astrocyte reactivity not only across disorders, but within the same disorder at different timepoints or in different CNS locations

For simulated ischemia/reperfusion (sI/R), ischemia was imposed by a buffer exchange to ischemic solution (20 mM HEPES, pH 6.6, 20 mM deoxyglucose, 125 mM NaCl, 8 mM KCl, 1.2 mM KH 2 PO 4 , 1.25 mM MgSO 4 , 1.2 mM CaCl 2 , 6.25 mM NaHCO 3 , 5 mM sodium lactate) and placing in a humidified chamber equilibrated with 95% N2 and 5% CO2

Overall survival (OS) and disease-free survival (DFS), defined as the time from operation to death and recurrence, respectively, were used as surrogates of prognosis

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doi: 10.1074/jbc.M507201200

- frequency of the intervention be reviewed if B12 levels are high-normal or elevated