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Dietary interactions between lysine and threonine in broilers

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May 30 2021;22(11)doi:10.3390/ijms22115885

Treatment of mutant lysates or supernatants with the reducing agent DTT (dithiothreitol) collapsed practically all the protein to monomeric size, suggesting that disulfide bonds, either intramolecular or intermolecular, are required for observation of multimers and larger species on denaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)

Lokole PB, Ngombe NK, Motomba DI, Safari JB, Mpuza MK, Krause RWM, et al

Brainina et al., 2019), developing physiologically relevant assay conditions (Lang et al., 2024), and implementing comprehensive approaches that combine multiple assessment methods