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Around 7000 individual chemicals have been identified in cigarette smoke 9 and many of these are linked to cardiovascular disease, respiratory disease, lung cancer and reproductive/developmental toxicity 10

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Bronchoalveolar Lavage (BAL) The animals were anesthetized with ketamine (150 mg/kg) and xylazine (10 mg/kg), and BAL was performed through a tracheal cannula with 3 1 mL phosphate-buffered saline (PBS) lavage

There were two barber chairs and a 1950s style Formica home bar counter that was four feet long

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