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Clean the injection site with alcohol, allow to dry, pinch the skin lightly if needed, insert the needle at a 45 to 90 degree angle depending on tissue depth, and inject slowly
injection of 50 mg/kg sodium pentobarbital (Sigma), and thoracic aorta was carefully dissected and maintained at 37 C in Krebs solution containing 118.2 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO 4 , 25 mM NaHCO 3 , 1.2 mM KH 2 PO 4 , 1.9 mM CaCl 2 and 11.7 mM dextrose (pH 7.4, 95% O 2 , 5% CO 2 )
Cell Physiol
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The peptide storage guide provides comprehensive handling recommendations