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NK cells were cultured and expanded in CTS NK-Xpander medium (A5019001, Gibco) supplemented with 5% human AB serum (H4522, Sigma-Aldrich) and 500 U/mL recombinant human IL-2 (HIL2-RO, Roche Diagnostics, Co., Ltd., Rotkreuz, Switzerland)

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Control + Rhodamine B (RB): Cells were treated with 1 M Rhodamine B in DMEM for 24 h under normoxic conditions

The caffeine kick was partly there, thanks to the extract and the L-theanine