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This method puts vitamin B12 into the fatty tissue under the skin

Moreover, recent experimental results show that two more histidine residues located in the C terminal region of PrP provide protection against neurotoxicity, when this is exerted from the N terminal region (Schilling et al., 2020)

For intracellular staining of phospho(p)-mTOR (Fisher Scientific 15549836) and p-S6 (eBioscience 15528216), cells were first stained for extracellular markers, fixed in 2% FA at RT, and stained for p-mTOR and p-S6 diluted 1:200 in saponin 5 g/mL

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