The cell pellet was resuspended in 50-100 L RIPA buffer [1% NP-40, 0.5% deoxycholate, 0.1% SDS, 150 mM NaCl, 50 mM Tris plus protease inhibitor cocktail (Sigma-Aldrich, P8340) and phosSTOP if phosphorylated proteins were being investigated (Sigma-Aldrich, 04906845001)]
Semaglutide joins liraglutide (Saxenda), which is another GLP-1 receptor agonist that was FDA-approved for weight management in 2014
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