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The samples were pooled and sequenced with a NextSeq 500 sequencing machine (Illumina) to give 61-bp paired-end reads

The gene expression data was analyzed for pathway analysis using a Cytoscape bioinformatics software 101 with a ClueGO plugin 102

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The meta-regression analysis suggested that the apparent protective association of GLP-1RAs may emerge relatively early in treatment, a finding consistent with randomized trials reporting improvements in walking capacity after short-term therapy

31.5% and 69.1% vs

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