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58 Additionally, G6PD activity can be regulated by several signaling pathways in tumors, such as the PI3K/AKT, Ras, Src, Nrf2, mTORC1, PETEN, ATM, and TP53 pathways, in a direct or indirect manner (reviewed in refs

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After a final wash step, a tetramethylbenzidine (TMB) peroxidase substrate solution (KPL Inc.) was added to each well and absorbance of the developed color was measured by a colorimetric plate reader (Molecular Device)

They consider factors such as hormonal imbalances , coexisting conditions, or persistent fatigue that may indicate underlying issues beyond weight