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Spiegelhalder, B., Eisenbrand, G

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After TMB substrate solution is added, only those wells that contain Human OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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By inhibiting guanylate cyclase, MB prevents NO from exerting its vasodilatory effect on blood vessels (since NOs relaxation of vascular smooth muscle is mediated by cyclic GMP)