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For each sample, a total of 10 pg of RNA was used to generate cDNA (SMARTer Ultra Low Input RNA v3 kit, Clontech) for library preparation

J Clin Endocrinol Metab (2015) 100(3):96876.10.1210/jc.2014-3144 144 SeimonRVBrennanIMRussoALittleTJJonesKLStandfieldSet alGastric emptying, mouth-to-cecum transit, and glycemic, insulin, incretin, and energy intake responses to a mixed-nutrient liquid in lean, overweight, and obese males

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Once you figure out that amount (which you can do by using Ros TDEE calculator ), you can then determine how much to cut your caloric intake to reach your goals safely