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Flag-HMGB1 or Flag-GSTP (WT), GSTP (S184A), and GSTP (S184D) were transfected into HEK293 cells, and then, the lysates were incubated with His-GSTP or His-HMGB1 immobilized on Ni 2+ IDAagarose beads (Novagen) in the lysis buffer at 4C for 8 h

Solubility: Not applicable (sterile aqueous solution miscible with most polar solvents)

For detection, 10 L of Reagent A and 20 L of Reagent B were sequentially added, and plates were incubated for 30 min at room temperature in the dark

In the present study, it is noted that the expression of ferritin (FTH1), a protein functioning in maintaining iron homeostasis, was significantly decreased at the protein level, indicating that riboflavin deficiency can impact significantly on iron absorption and utilization 48,49

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