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The ESI source conditions were set as following: sheath gas flow rate of 50 arbitrary units (Arb), auxiliary gas flow rate of 15 Arb, capillary temperature of 320 C, full MS resolution of 60,000, MS/MS resolution of 15,000, collision energy of 10/30/60 in normalized collision energy (NCE) mode, and spray voltage of 3.8 kV (positive) or 3.4 kV (negative)

That controlling point is also recognized in the most recent reviews (Gwyer et al., 2019

33 Given these substantial beneficial effects on cardiometabolic risk factors, it is unsurprising that incretins have also been shown to reduce the risk of cardiometabolic diseases