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also showed muscle atrophy was more pronounced after ablation of muscle IGF-1 production than when hepatic IGF-1 production was suppressed (Bikle et al., 2000

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The cells were washed twice with PBS, trypsinized, and then stained with propidium iodide(1 mg/mL)

doi: 10.1152/ajpheart.00346.2019 180 TangTWChenH-CChenC-YYenCYLinC-JPrajnamitraRPet al