In South Carolina, the Clean Indoor Air Act was passed which prohibits smoking of any kind in all indoor public areas except where there is a designated smoking area (US Legal, 2022)
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However, recombinant expression of cardosin A and B in tobacco leaves revealed accumulation of these proteins in the vacuole and the presence of two vacuolar sorting signals located at the PSI and C-terminal of the protein 9,12,19
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