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Age and sex effects on plasma metabolite association networks in healthy subjects

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Insoluble material was removed by centrifugation at 65,000 g for 40 min and the solubilized complex was incubated overnight at 4 C with pre-equilibrated Nickel resin (Ni Smart Beads 6FF, SMART Lifesciences) containing 10 mM imidazole

The interplay among ROS, antioxidant networks, and cell death pathways reveals a complex yet promising landscape

Many also fail to document their process thoroughly, making it difficult to troubleshoot or reproduce results