Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure
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doi: 10.1016/j.biopha.2019.109249 22 ZiaAFarkhondehTPourbagher-ShahriAMSamarghandianS
C), 38.77 (CH 2 ), 38.35 (CH 2 ), 27.50 (CH 2 ), 26.84 (d, J = 16.7 Hz, CH 2 ), 22.37 (d, J = 51.3 Hz, CH 2 ), 21.77 (d, J = 4.3 Hz, CH 2 )
Because the cysteine thiol oxidises to the disulfide GSSG on air exposure, keep stopper-open time and headspace to a minimum and avoid alkaline solvents, which increase thiolate character and accelerate oxidation