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HeLa and HEK-293T cells were maintained in either DMEM media (Gibco) containing 4.5g/L glucose, 110mg/L pyruvate, 4mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin or in RPMI media (Gibco) containing 2 mM glutamine, 10% fetal bovine serum, 1% penicillin and streptomycin