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Urinary metabonomics study of anti-depressive mechanisms of Millettia speciosa Champ on rats with chronic unpredictable mild stress-induced depression

These are hypothesis-generating, not proof

2.8 Pull-down experiments Chloroplast extracts (100 g of Chl) were solubilized with 1% (w/v) dodecyl maltoside (DM) for 10 min at 4C, and incubated with 50 g of either purified His-TRX-f1, His-TRX-m1 and His-NTRC as bait proteins in binding buffer (BF, 25 mM Tris-HCl [pH 7.8], 150 mM NaCl, 5 mM MgCl 2 , 10% [v/v], glycerol, and cOmplete protease inhibitor [Roche]) overnight at 4C and 45 rpm

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6, Tnf- , Il-6 , Il-1 , Il-1 , Il-17 , Il-22 , and Il-23 was significantly decreased in the IMQ-Fer-1 group compared to those of the IMQ group (Fig