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There are reports of increased VEGF (linked to better blood supply) and modulation of TGF1, a cytokine associated with follicle regression and androgendriven thinning [1]

You cannot eliminate dissolved oxygen entirely, but you can minimize exposure by keeping the vial sealed (minimizing headspace), storing it upright (reducing the air-liquid interface), and using the solution within the 28-day window

Its recommended not to freeze (as mentioned) and also to avoid excessive heat

Tendon fibroblasts between passages 2 and 4, having proper growth rate and normal fibroblast shape, were used in the following experiments

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