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6 The 5% TCA2% 2-mercaptoethanol extracts (50 L) were dried under vacuum to remove excess amounts of 2-mercaptoethanol

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Materials and Methods Reagents and Cell Culture The compound selected from virtual screen was purchased from SPECS with purity of more than 95% (confirmed by SPECS with NMR and LC-MS

What you will need: A vial of lyophilized peptide A vial of bacteriostatic water An insulin syringe (typically 1 ml / 100 IU) or a precise pipette Disinfectant swabs (70% isopropyl alcohol) A refrigerator for storage Procedure: Let both vials reach room temperature