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She even complained about having to make the call on her lunch break (a call time that I did not sent myself.) My concern is that is the nurse is this aggressive on the phone, what must she be like if you have questions or concerns during your treatment

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The total RNA was reverse transcribed into cDNA and analyzed using SYBR quantitative PCR (qPCR) with the following cycling conditions: 10 min of denaturation at 95C, followed by 40 cycles of 30 s at 95C and 30 s at 60C