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The primary antibodies were visualized using goat anti-rabbit Alexa Fluor 555 conjugate and goat anti-mouse Alexa Fluor 488 conjugate antibodies (1:800) (Cell Signaling Technology)

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This process is also typically accompanied by the inhibition of glutathione peroxidase 4 (GPX4) and glutathione (GSH) systems, which normally eliminate lipid peroxides [7, 8]

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