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The siRNA sequences were as follows: siRNA-GSDMD-1: 5-GCCAGAACACAAAGTCCTGCA-3, siRNA-GSDMD-2: 5-GAGCTTCCACTTCTACGATGC-3, siRNA-GSDMD-3: 5-GCTGGTTATTGACTCTGACTT-3, nonsense siRNA: 5-TTCTCCGAACGTGTCACGT-3

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A longer follow-up period might provide better discernment of differences in HCRU, and this should be considered for future studies

Injection site reactions: redness, itching or mild pain occur occasionally

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