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Although current results from human trials are limited, existing research suggests that probiotics can influence 25-hydroxy VitD levels, calcium intake and absorption, and slightly reduce bone loss in elderly PMW, with effects comparable to calcium and VitD supplementation ( Bifidobacterium animalis subsp

The quality and concentration of DNA were determined by 1.0% agarose gel electrophoresis and a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, MA, USA)

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