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Medium from each of the four wells was collected and stored at 80 C prior to immunoassays

Timing considerations suggest applying topical GHK-Cu at different times than other active skincare ingredients

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The overall impression from available evidence is that DSIP appears reasonably well-tolerated with a low incidence of serious adverse effects in the populations and contexts studied, but significant gaps in safety knowledge remain, particularly regarding long-term use, use in specific populations (elderly, children, those with comorbidities), and comprehensive toxicology assessment meeting modern pharmaceutical standards

For general wellness, one shot per month may be sufficient