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When comparing metabolic parameters pre and post the 2-week treatment, we observed that liraglutide significantly lowered fat mass in male WT mice, however similar changes failed to reach statistical significance in female WT mice ( Figure 2E )

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For comparisons among three or more independent groups, one-way analysis of variance (ANOVA) followed by Tukeys multiple comparisons test was applied when variances were equal, whereas Welchs ANOVA with Dunnetts T3 post-hoc test was used when variances were unequal

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The molds were then centrifuged again for 5 min