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[DOI] [Google Scholar] 48.Konuspayeva G, Serikbayeva A, Loiseau G, Narmuratova M, Faye B
The decreased malate/pyruvate and GSH levels with LbNOX expression strongly suggest a decreased cytoplasmic (free) [NADPH]/[NADP + ], while the large increase in pyruvate also strongly suggests that the pyruvate/lactate, a proxy for the cytoplasmic (free) [NAD + ]/[NADH], increased, which likely drove the lifespan extension in the females
And a crucial point: avoid repeated freeze-thaw cycles with the liquid solution